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BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families. There are several types of BLAST searches.
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The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. four different kinds of the BLAST program can be run: BLASTP, Protein Query Searching a Protein Database Each database sequence is compared to each query in a separate protein-protein pairwise comparison. BLASTX, Nucleotide Query Searching a Protein Database Each query is translated, and each of the six products is compared to BLAST stands for Basic Local Alignment Search Tool. The emphasis of this tool is to find regions of sequence similarity, which will yield functional and evolutionary clues about the structure and function of your sequence.
Standard Nucleotide BLAST BLASTN programs search nucleotide databases using a nucleotide query. more... This title appears on all BLAST results and saved searches. Enter one or more queries in the top text box and one or more subject sequences in the lower text box. Then use the BLAST button at the bottom of the page to align your sequences. Welcome to BLAST Help ¶ Getting started ¶ Web BLAST quick start guide New BLAST databases New Guide to BLAST home and search pages Blast report description Blast topics Frequently Asked Questions Getting Help ¶ Write to the help desk Mailing list BLAST tutorials About BLAST ¶ NCBI Handbook The Statistics of Sequence Similarity Scores BLAST ... The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between protein or nucleotide sequences. The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches. Smart Blast searches a protein query against the landmark database Enter Protein Query Sequence
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